Cloning of rat hepatic lipase cDNA: evidence for a lipase gene family.
AUTOR(ES)
Komaromy, M C
RESUMO
Clones for rat hepatic lipase were isolated by probing a rat liver cDNA library in lambda gt11 with an oligonucleotide synthesized on the basis of a partial peptide sequence. The cloned messenger codes for a protein of 472 amino acids plus a hydrophobic leader sequence of 22 amino acids. The unglycosylated protein has a predicted molecular weight of 53,222 and contains two potential sites for N-glycosylation. The protein bears striking regions of homology with other known lipases and contains peptide sequences that have been implicated in lipid binding. The homologous mRNA is present in liver tissue but no detectable mRNA is observed in the adrenal gland, despite the reported presence of hepatic lipase in both the liver and the adrenal gland. No mRNA was seen in any of a variety of other tissues.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=304467Documentos Relacionados
- Molecular cloning of cDNA for the nuclear ribonucleoprotein particle C proteins: a conserved gene family.
- Isolation of cDNA clones encoding protein kinase C: evidence for a protein kinase C-related gene family.
- Molecular cloning of human gastrin cDNA: evidence for evolution of gastrin by gene duplication.
- Cloning and sequencing of a full length cDNA coding for a human apoferritin H chain: evidence for a multigene family.
- Isolation and characterization of cDNA clones encoding human liver glutamate dehydrogenase: evidence for a small gene family.
