Cloning and expression of a human voltage-gated potassium channel. A novel member of the RCK potassium channel family.

AUTOR(ES)

Grupe, A

RESUMO

We have isolated and characterized a human cDNA (HBK2) that is homologous to novel member (RCK2) of the K+ channel RCK gene family expressed in rat brain. RCK2 mRNA was detected predominantly in midbrain areas and brainstem. The primary sequences of the HBK2/RCK2 K+ channel proteins exhibit major differences to other members of the RCK gene family. The bend region between segments S1 and S2 is unusually long and does not contain the N-glycosylation site commonly found in this region. They might be O-glycosylated instead. Functional characterization of the HBK2/RCK2 K+ channels in Xenopus laevis oocytes following micro-injection in in vitro transcribed HBK2 or RCK2 cRNA showed that the HBK2/RCK2 proteins form voltage-gated K+ channels with novel functional and pharmacological properties. These channels are different to RCK1, RCK3, RCK4 and RCK5 K+ channels.

Documentos Relacionados

• A voltage-gated potassium channel in human T lymphocytes.
• VKCDB: Voltage-gated potassium channel database
• Mechanism of charybdotoxin block of a voltage-gated K+ channel.
• Overview of the voltage-gated sodium channel family
• Molecular cloning of an atypical voltage-gated sodium channel expressed in human heart and uterus: evidence for a distinct gene family.