Cloning and characterization of small circular DNA from Chinese hamster ovary cells.
AUTOR(ES)
Stanfield, S W
RESUMO
Small polydisperse circular (spc) DNA was isolated and cloned, using BglII from Chinese hamster ovary (CHO) cells. The properties of 47 clones containing at least 43 different BglII fragments are reported. The majority of the clones probably contain entire sequences from individual spcDNA molecules. Most of the clones were homologous to sequences in CHO cell chromosomal DNA, and many were also homologous to mouse LMTK- cell chromosomal sequences. The majority of homologous CHO cell chromosomal sequences were repetitive, although a few may be single copy. Only a small fraction of cloned spcDNA molecules were present in every cell; most occurred less frequently than once in 15 cells. Localization studies indicated that at least a portion of spcDNA is associated with the nucleus in CHO cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=368672Documentos Relacionados
- Multiple mechanisms generate extrachromosomal circular DNA in Chinese hamster ovary cells.
- Characterization of a mutator gene in Chinese hamster ovary cells.
- Molecular cloning and characterization of small polydisperse circular DNA from mouse 3T6 cells.
- Imaging of luciferase secretion from transformed Chinese hamster ovary cells.
- Cloning of cDNA encoding a 100 kDa nucleolar protein (nucleoline) of Chinese hamster ovary cells.