Clonagem, expressão e purificação de proteínas candidatas vacinais de Leptospira interrogans sorovar Copenhageni. Avaliação de atividades imunogênicas e características funcionais. / Cloning, expression and purification of proteins, vaccine candidates from Leptospira interrogans sorovar Copenhageni. Evaluation of immunogenic and functional characteristics.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Leptospirosis is a zoonosis worldwide distributed, caused by bacteria from the genus Leptospira. The development of an efficient vaccine of sub-unities would be of major interest for public health. We proposed to investigate 4 antigens from Leptospira interrogans srv Copenhageni, genes LIC12659, LIC12660, LIC12631 and LIC10868, which were amplified and cloned into expression vectors pAE and pAEsox. The proteins VapB, VapC, VapBC and Sph2 were expressed in E. coli and in salmonella SL3261, and purified by metal-affinity chromatography. The activities hemolytic of Sph2 and ribonuclease of VapC were not confirmed, possibly due to differences between native and recombinant proteins. The clones of E. coli with genes of the toxin-antitoxin module vapBC suffered inhibition (vapC) and recovery (vapBC) of growth rate as described. The recombinant proteins VapB purified or in salmonella and purified Sph2, showed to be immunogenic in mice and in hamsters. The immunization with Salmonella-VapB determined better protection on challenge assay.

ASSUNTO(S)

salmonella leptospirosis leptospirosis animal leptospirose leptospirose animal biotechnology antigens of bacteria vaccines. vacinas biotecnologia salmonella antígenos de bactérias

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