Clonagem, expressão e avaliação da imunogenicidade e do potencial adjuvante induzidos pela proteína "heat-shock"  Cpn60 da Bordetella pertussis. / Molecular cloning, expression and evaluation of immunogenicity and adjuvant potential induced from the heat-shock protein Cpn60 from Bordetella pertussis.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

The Cpn60 protein is a member of a group of higly conserved proteins linked to essencial cell functions. The Cpn60 was cloned, expressed and its immune response has been evaluated. BALB\c mice were immunized with 5 or 10 µg of the recombinant protein (Cpn60r) alone or mixed with DPT vaccine without aluminum hidroxyde (NADPT). The DPT vaccine from Instituto Butantan was used as control. We evaluated the cytokines production by spleen cells after they have been reestimulated in vitro with Cpn60r. The animals were challenged after the immunization protocol. The Cpn60r alone or mixed with NADPT vaccine was able to induce higher antibodies levels than DPT. IgG1 and IgG2a levels were similar in all groups. We could detect levels of IL-6 and IFN-γ on groups immunized with Cpn60r. The groups immunized with Cpn60r+NADTP showed a 60 and 80% protection rate against the challenge with the live bacteria, similar to the group immunized with DPT. These results show the immune response of the recombinant protein that could be included in immunization protocols for pertussis.

ASSUNTO(S)

gram-negative aerobic bacteria vacinas clonagem molecular molecular cloning adjuvantes imunológicos coqueluche heat-shock proteins immunologic adjuvants vaccines whooping cough proteínas do choque térmico bactérias aeróbicas gram-negativas

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