Cleavage of Adenovirus Type 2 DNA into Six Unique Fragments by Endonuclease R·RI
The DNA of adenovirus type 2 was cleaved by restriction endonuclease R·RI into six fragments. These fragments were separated by electrophoresis on composite agarose-polyacrylamide gels. Their molecular weights ranged from 1.1 × 106 to 13.6 × 106, as measured by electron microscopy. Each fragment represented a unique segment of adenovirus type 2 DNA since: (i) the fragments were obtained in equimolar amounts; (ii) the sum of their molecular weights was equal to the molecular weight of complete adenovirus DNA; and (iii) each fragment exhibited a rate of renaturation that was inversely proportional to its size.
ACESSO AO ARTIGOhttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=433215
- Physical map and strand polarity of specific fragments of adenovirus-associated virus DNA produced by endonuclease R-EcoRI.
- Kinetic studies on the cleavage of adenovirus DNA by restriction endonuclease Eco RI.
- Cleavage of Replicating Forms of Mitochondrial DNA by EcoRI Endonuclease
- Analysis of Endonuclease R·EcoRI Fragments of DNA from Lambdoid Bacteriophages and Other Viruses by Agarose-Gel Electrophoresis
- A Colinear Map Relating the Simian Virus 40 (SV40) DNA Segments of Six Adenovirus-SV40 Hybrids to the DNA Fragments Produced by Restriction Endonuclease Cleavage of SV40 DNA