Citomegalovirus em transplantados renais : diagnostico pela reação em cadeia da polimerase (PCR) e impacto clinico

AUTOR(ES)
DATA DE PUBLICAÇÃO

1993

RESUMO

Cytomegalovirus (C.M.V.) is the single most important infections agent affecting recipients of organ transplant. To evaluate the incidence and the clinical impact of cytomegalovirus infection on renal transplant in Brazil, 37 patients who underwent renal allograft transplants were periodically screened for the presence of C.M.V. virus DNA in urine by lIsing polymerase chain reaction (P.C.R.) and for the presence of IgM and IgG cytomcgalovirlls antibodies (ELlSA and I.F.I.). The P.C.R. amplificd prodllcts werc detected by gel electrophoresis and by dot blot hybridization with oligonucleotide probes. 32 out 37 patients (86.48%) were found to be positive by at least one of the three methods. In 6 patients, P.C.R. was the only test to detect the C.M.V. infection. 10 patients had a positive result by P.C.R.Qefore the transplantation. The diagnosis was made earlier by P.C.R. than by serologic tests. Active infection occured more frequently in the first 4 months after transplantation. 16 out 32 patients (50.00%) with active infection by C.M.V. presented clinical manifestations of C.M. V. disease. 5 patients without evidences of active infection by the 3 tests have only minor clinical complications during followup. Our results sugest that C.M.V. infection is a frequent problem in renal transplant patients in Brazil, with significant clinical complications. Moreover, we confirmed that P.C.R. is a very sensitive procedure to early detection of C.M.V. infection

ASSUNTO(S)

rins - transplante - infecção virus

ACESSO AO ARTIGO

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