cis-Acting Signals in Encapsidation of Hantaan Virus S-Segment Viral Genomic RNA by Its N Protein
AUTOR(ES)
Severson, William E.
FONTE
American Society for Microbiology
RESUMO
The nucleocapsid (N) protein encapsidates both viral genomic RNA (vRNA) and the antigenomic RNA (cRNA), but not viral mRNA. Previous work has shown that the N protein has preference for vRNA, and this suggested the possibility of a cis-acting signal that could be used to initiate encapsidation for the S segment. To map the cis-acting determinants, several deletion RNA derivatives and synthetic oligoribonucleotides were constructed from the S segment of the Hantaan virus (HTNV) vRNA. N protein-RNA interactions were examined by UV cross-linking studies, filter-binding assays, and gel electrophoresis mobility shift assays to define the ability of each to bind HTNV N protein. The 5′ end of the S-segment vRNA was observed to be necessary and sufficient for the binding reaction. Modeling of the 5′ end of the vRNA revealed a possible stem-loop structure (SL) with a large single-stranded loop. We suggest that a specific interaction occurs between the N protein and sequences within this region to initiate encapsidation of the vRNAs.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=115888Documentos Relacionados
- Location of cis-acting signals important for RNA encapsidation in the leader sequence of human immunodeficiency virus type 2.
- cis-acting sequences required for encapsidation of duck hepatitis B virus pregenomic RNA.
- Characterization of the cis-Acting Contributions to Avian Hepadnavirus RNA Encapsidation
- A cis-acting DNA signal for encapsidation of simian virus 40.
- Mutational analysis of cis-acting packaging signals in human immunodeficiency virus type 1 RNA.