Chromosome-mediated gene transfer of multidrug resistance.
AUTOR(ES)
Gros, P
RESUMO
Multidrug resistance can be transferred from drug-resistant LZ Chinese hamster cells to drug-susceptible mouse LTA cells by chromosome-mediated gene transfer. Analysis of genomic DNA demonstrated the transfer of multiple copies of a DNA domain which is amplified in the donor multidrug-resistant cells. The transfer of 10 to 15 copies of the Chinese hamster gene was sufficient to produce a multidrug-resistant phenotype. Chromosome transferents exhibited overexpression of an mRNA of approximately 5 kilobases which has previously been demonstrated to be encoded by the amplified DNA domain of the donor LZ cells. Phenotypic analysis of individual clones selected in adriamycin showed the resistance to be pleiotropic. All clones tested demonstrated similar levels of cross-resistance to the drugs daunorubicin and colchicine. These results indicate that the DNA sequences transferred confer the complete multidrug-resistant phenotype on recipient cells and suggest that multidrug resistance is due to overexpression of the protein encoded by the 5-kilobase mRNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=367139Documentos Relacionados
- Molecular analysis of chromosome-mediated gene transfer.
- Chromosome-mediated transfer of the murine Na,K-ATPase alpha subunit confers ouabain resistance.
- Chromosome-mediated gene transfer of hydroxyurea resistance and amplification of ribonucleotide reductase activity.
- Chromosome-mediated gene transfer results in two classes of unstable transformants.
- Chromosome-mediated gene transfer between closely realted strains of cultured mouse cells.
