Chlamydia trachomatis-specific antibodies in patients with pelvic inflammatory disease: comparison with isolation in tissue culture or detection with polymerase chain reaction.
AUTOR(ES)
Theunissen, J J
RESUMO
OBJECTIVE--The detection of acute phase antibodies against C trachomatis and its comparison with tissue culture or polymerase chain reaction (PCR) on samples of cervix and urethra obtained from patients with pelvic inflammatory disease (PID). METHODS--In the academic hospital Dijkzigt, Rotterdam, The Netherlands, prospective investigations were performed on 49 consecutive patients who were admitted with the diagnosis of PID. Infections with C trachomatis were traced using tissue culture, PCR or by determining acute phase IgG and IgM antibodies. Differences between the sensitivities of serology and tissue culture or PCR were calculated using the Fisher exact test. RESULTS--C trachomatis infection was detected more often in PID patients using serology in comparison with PCR (p < 0.05) or tissue culture (p < 0.05). All patients who were positive in tissue culture also had acute phase antibodies. CONCLUSION--The establishing of acute C trachomatis infections in PID patients on the basis of serology was superior to either tissue culture or PCR on samples obtained from cervix and urethra.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1195270Documentos Relacionados
- Detection of Chlamydia trachomatis DNA in patients with non-gonococcal urethritis using the polymerase chain reaction.
- Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction.
- Sensitive detection and typing of Chlamydia trachomatis using nested polymerase chain reaction.
- Quantitation of Chlamydia trachomatis by culture, direct immunofluorescence and competitive polymerase chain reaction.
- Antigen specific serum antibody response to Chlamydia trachomatis in patients with acute pelvic inflammatory disease.
