Chicken lens delta-crystallin gene expression and methylation in several non-lens tissues.

AUTOR(ES)

Bower, D J

RESUMO

RNA sequences coding for the most abundant chicken lens proteins, delta-crystallin, were detected at very low levels in day old post hatch chick lung, heart, kidney and liver, and in 6 day embryo headless bodies. The pattern of cytosine methylation within the CCGG sequences of the delta-crystallin genes was also examined and shown to vary in several non-lens tissues, from several stages of development. Embryonic neural retina, which expresses a higher level of delta-crystallin RNA than the above tissues, is no less methylated in the sites studied than the tissues which have no association with the eye, and is actually more heavily methylated than the kidney. Thus no obvious correlation was found between undermethylation and gene expression.

Documentos Relacionados

• The pattern of expression of chick delta-crystallin genes in lens differentiation and in trans-differentiating cultured tissues.
• Nucleotide sequence of a chicken delta-crystallin gene.
• Delta-crystallin genes become hypomethylated in postmitotic lens cells during chicken development.
• Developmental regulation of hypomethylation of delta-crystallin genes in chicken embryo lens cells.
• Organization of delta-crystallin genes in the chicken.