Chi18A, the Endochitinase in the Cellulosome of the Thermophilic, Cellulolytic Bacterium Clostridium thermocellum
AUTOR(ES)
Zverlov, Vladimir V.
FONTE
American Society for Microbiology
RESUMO
The chitinase gene chiA was identified on the Clostridium thermocellum genome downstream of the endoglucanase gene celA. It contains a catalytic module of glycosyl hydrolase family 18 and a cellulosomal dockerin module. Chi18A hydrolyzes aryl-acetyl-chito-oligosaccharides preferentially. In denaturing electrophoresis of purified cellulosomes, a single chitinase activity band was identified in zymograms and Western blots, indicating that Chi18A is the only chitinase in the cellulosome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=123980Documentos Relacionados
- Comparison of Cellulolytic Activities in Clostridium thermocellum and Three Thermophilic, Cellulolytic Anaerobes
- Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum.
- Chemically Defined Medium for Growth of Clostridium thermocellum, a Cellulolytic Thermophilic Anaerobe
- Organization and distribution of the cellulosome in Clostridium thermocellum.
- Involvement of Both Dockerin Subdomains in Assembly of the Clostridium thermocellum Cellulosome
