Chemical Characterization and Optimization of the Extraction Process of Bioactive Compounds from Propolis Produced by Selected Bees Apis mellifera

AUTOR(ES)

Oldoni, Tatiane Luiza Cadorin, Oliveira, Sheila C., Andolfatto, Suelen, Karling, Mariéli, Calegari, Matheus A., Sado, Ricardo Y., Maia, Fabiana M. C., Alencar, Severino M., Lima, Vanderlei A.

FONTE

J. Braz. Chem. Soc.

DATA DE PUBLICAÇÃO

2015-10

RESUMO

The extraction process of bioactive compounds from propolis produced by selected bees was optimized using a 2³ factorial design planning to evaluate the effect of ethanol concentration, time and temperature on the extraction of total phenolic compounds and antioxidant activity. Analysis of total flavonoids, antioxidant activity and chemical characterization performed by high-performance liquid chromatography with photodiode array (HPLC-PDA) detection were carried out in optimal conditions of extraction. Optimizing results suggest that the best condition for extraction of bioactive compounds was obtained in higher levels: time (45 min), temperature (70 °C) and concentration of ethanol (80%). The extract evaluated in optimal conditions presented good antioxidant activity by the radical scavenging 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and ferric reducing antioxidant power (FRAP) methods and chromatographic analysis identified phenolic acids: para-coumaric, ferulic and caffeic, indicating that this material is a potential source of bioactive compounds.

Documentos Relacionados

• CHEMICAL COMPOSITIONS AND ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF PROPOLIS PRODUCED BY Frieseomelitta longipes AND Apis mellifera BEES
• The antibacterial activity of propolis produced by Apis mellifera L. and Brazilian stingless bees
• Hepatoprotective and antineoplastic potencial of red propolis produced by the bees Apis mellifera in the semiarid of Rio Grande do Norte, Brazil
• Isolamento e identificação de compostos com atividade antioxidante de uma nova variedade de própolis brasileira produzida por abelhas da espécie Apis mellifera
• Optimizing the supercritical fluid extraction process of bioactive compounds from processed tomato skin by-products