Chemical and technological evaluation of the Phyllanthus niruri aerial parts as a function of cultivation and harvesting conditions

AUTOR(ES)

Couto, Angélica G., Kunzler, Marli L., Spaniol, Bárbara, Magalhães, Pedro M., Ortega, George G., Petrovick, Pedro R.

FONTE

Rev. bras. farmacogn.

DATA DE PUBLICAÇÃO

01/02/2013

RESUMO

Tea from Phyllanthus niruri L., Phyllanthaceae, aerial parts is commonly used by Brazilian folk medicine for its benefits on the treatment of genitourinary disorders, for what the polyphenolic compounds are mainly responsible. The yield of such compounds may be influenced by several variables related with the plant growth. The effects of planting periods and harvesting conditions are investigated in this work, including four different seasons. The cultivation was characterized by dry mass yield of aerial parts, and the effect of pruning was analyzed. Leaves, stems and their mixtures were analyzed after drying and milling. Loss on drying and water soluble extractives were used as physical parameters for quality control. Flavonoid content and gallic acid were chosen as chemical markers for this work. The spectrophotometric trial based on the aluminum chloride complexes was applied to evaluate the total flavonoids content. Gallic acid contents were measured from the water extractive solutions by high-performance liquid chromatography. The pruning caused a positive influence on the amount of leaves and stems. The highest flavonoids and gallic acid contents were found in the leaves, which were developed over the summer and the winter, respectively, both from the second harvesting (after pruning). Chomatographic profile by HPLC was characterizes by the presence of gallic acid and two other major peaks (not identified substances), which relation was peculiar to each aerial part. In conclusion, these results suggest that even under less favorable climatic conditions, in winter, the pruning seems to cause a strong influence over the P. niruri polyphenolics production. Indeed, the total flavonoids content, as well as the HPLC profile, can be used as indicative parameters of the ratio of leaves and stem in the vegetal raw material.

Documentos Relacionados

• Phyllanthus niruri as a promising alternative treatment for nephrolithiasis
• Desenvolvimento tecnológico de soluções extrativas aquosas de Phyllanthus niruri L. (quebra-pedra) empregando planejamento fatorial
• Evaluation of (anti)genotoxic activities of Phyllanthus niruri L. in rat bone marrow using the micronucleus test
• Estudo comparativo de trÃs espÃcies de Phyllanthus (Phyllanthaceae) conhecidas por quebra-pedra (Phyllanthus niruri L., Phyllanthus amarus Schum &Thonn. e Phyllanthus tenellus Roxb.)
• Chemical Constituents, Antiproliferative and Antioxidant Activities of Vernonanthura nudiflora (Less.) H. Rob. Aerial Parts