Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila.
AUTOR(ES)
Feeley, J C
RESUMO
Charcoal-yeast extract agar is a new bacteriological medium that supports excellent growth of the Legionella pneumophila. It results from modifications made in an existing L. pneumophila medium, F-G agar. Yeast extract, instead of an acid hydrolysate of casein, serves as the protein source. Beef extractives and starch are not added. Activated charcoal (Norit A or Norit SG) is included at 0.20% (wt/vol). Comparison of charcoal-yeast extract and F-G agars showed that a greater number of colony-forming units of L. pneumophila was recovered from a standardized tissue inoculum on charcoal-yeast extract agar (4.35 x 10(6) colony-forming units) than on F-G agar (4.85 x 10(4) colony-forming units). Macroscopic colonies of L. pneumophila were visible on the new medium within 3 days, whereas 4 days of growth was required on F-G agar.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=273193Documentos Relacionados
- Buffered charcoal-yeast extract medium for the isolation of brucellae.
- Isolation of Nocardia asteroides on buffered charcoal-yeast extract agar.
- Aniline blue-containing buffered charcoal-yeast extract medium for presumptive identification of Legionella species.
- Selective buffered charcoal-yeast extract medium for isolation of nocardiae from mixed cultures.
- Use of selective buffered charcoal-yeast extract medium for isolation of nocardiae from mixed cultures.