Charaterization of Leishmania major Friedlin Telomeric Terminus

AUTOR(ES)

Chiurillo, Miguel Angel, Ramírez, José Luis

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2002-04

RESUMO

Here we have characterized Leishmania major (Friedlin) telomeric terminus (the very end) using recombinants obtained by a vector-adaptor cloning protocol. As in L. donovani, the last nine nucleotides of L. major terminus are 5'-GGTTAGGGT-OH 3', differing from Trypanosoma cruzi and T. brucei terminus 5'GGGTTAGGG-OH 3', thus indicating that these sequences are genus specific. We have also made a comparative analysis between L. major and L. donovani telomere-associated sequences, and described a novel non-repeated telomeric associated sequence common to L. major low molecular weight chromosomal bands.

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