Characterization of the human spr2 promoter: induction after UV irradiation or TPA treatment and regulation during differentiation of cultured primary keratinocytes.
AUTOR(ES)
Gibbs, S
RESUMO
We have isolated genomic clones from several members of the UV and TPA inducible human spr2 gene-family in order to analyse the regulation of these genes at a molecular level. From one of these members, the spr2-1 gene, we have identified and sequenced the regulatory region. By using CAT fusion plasmids and a liposome mediated transfection procedure we show that the isolated promoter region contains all the cis-elements necessary for induced expression after UV irradiation or phorbolester treatment of cultured human keratinocytes. Additionally the spr2-1 promoter is shown to be regulated aswell during the normal process of keratinocyte differentiation. This makes the spr2-1 promoter sequence an ideal tool to study the molecular mechanisms by which environmental agents such as UV radiation and chemical tumor promoters interfere with normal gene expression during cell proliferation and differentiation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=331257Documentos Relacionados
- Effects of UV, 4-NQO and TPA on gene expression in cultured human epidermal keratinocytes.
- Thrombomodulin expression by human keratinocytes. Induction of cofactor activity during epidermal differentiation.
- Regulation of the human papillomavirus type 11 E6 promoter by viral and host transcription factors in primary human keratinocytes.
- Methotrexate induces differentiation of human keratinocytes.
- Epidermal differentiation and dermal changes in healing following treatment of surgical wounds with sheets of cultured allogeneic keratinocytes.
