Characterization of the genome of the Rhodococcus rhodochrous bacteriophage NJL.
AUTOR(ES)
Sunairi, M
RESUMO
Temperate bacteriophage NJL of Rhodococcus rhodochrous has a 49-kb linear double-stranded DNA with cohesive ends (cos). NJL DNA has unique target sites for HindIII and SspI, two target sites each for NheI and ScaI, and no cleavage site for AxyI, DraI, EcoRI, SacI, and SphI. The single-stranded regions of cos ends were ligated to each other with T4 DNA ligase, removed with mung bean nuclease, or blunted with the Klenow large fragment of DNA polymerase I; then the sequences of the cos ends were determined. Comparison of these sequences revealed that the single-stranded regions are complementary and 18 bases long and protrude at the 3' ends; they have the following sequences: 5'-TTGGCACCGTGGGAGGAG-3' and 3'-AACCGTGGCAC CCTCCTC-5'. A physical map of NJL was constructed by a cos mapping method based on information about the structure of the cohesive ends and multiple digestions with restriction endonucleases.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=202061Documentos Relacionados
- Spectroscopic characterization of a newly isolated cytochrome P450 from Rhodococcus rhodochrous.
- Metabolism of 2-Mercaptobenzothiazole by Rhodococcus rhodochrous
- Metabolism of styrene by Rhodococcus rhodochrous NCIMB 13259.
- Novel Pathway for Utilization of Cyclopropanecarboxylate by Rhodococcus rhodochrous
- Purification and characterization of a novel nitrilase of Rhodococcus rhodochrous K22 that acts on aliphatic nitriles.
