Characterization of the Antirrhinum floral homeotic MADS-box gene deficiens: evidence for DNA binding and autoregulation of its persistent expression throughout flower development.
AUTOR(ES)
Schwarz-Sommer, Z
RESUMO
We have determined the structure of the floral homeotic deficiens (defA) gene whose mutants display sepaloid petals and carpelloid stamens, and have analysed its spatial and temporal expression pattern. In addition, several mutant alleles (morphoalleles) were studied. The results of these analyses define three functional domains of the DEF A protein and identify in the deficiens promoter a possible cis-acting binding site for a transcription factor which specifically upregulates expression of deficiens in petals and stamens. In vitro DNA binding studies show that DEF A binds to specific DNA motifs as a heterodimer, together with the protein product of the floral homeotic globosa gene, thus demonstrating that the protein encoded by deficiens is a DNA binding protein. Furthermore, Northern analysis of a temperature sensitive allele at permissive and non-permissive temperatures provides evidence for autoregulation of the persistent expression of deficiens throughout flower development. A possible mechanism of autoregulation is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=556446Documentos Relacionados
- DNA binding and dimerisation determinants of Antirrhinum majus MADS-box transcription factors.
- Characterization of MADS-box genes in charophycean green algae and its implication for the evolution of MADS-box genes
- Molecular Evolution of Flower Development: Diversification of the Plant Mads-Box Regulatory Gene Family
- DNA binding specificity determinants in MADS-box transcription factors.
- DNA binding by MADS-box transcription factors: a molecular mechanism for differential DNA bending.