Characterization of Shigella spp. by antimicrobial resistance and PCR detection of ipa genes in an infantile population from Porto Velho (Western Amazon region), Brazil
AUTOR(ES)
Silva, Tatiane, Nogueira, Paulo Afonso, Magalhães, Gleiciene Félix, Grava, Andréa Fagundes, Silva, Luiz Hildebrando Pereira da, Orlandi, Patrícia Puccinelli
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2008-11
RESUMO
The incidence of Shigella spp. was assessed in 877 infants from the public hospital in Rondônia (Western Amazon region, Brazil) where Shigella represents the fourth cause of diarrhea. Twenty-five isolates were identified: 18 were Shigella flexneri, three Shigella sonnei, three Shigella boydii and one Shigella dysenteriae. With the exception of S. dysenteriae, all Shigella spp. isolated from children with diarrhea acquired multiple antibiotic resistances. PCR detection of ipa virulence genes and invasion assays of bloody diarrhea and fever (colitis) were compared among 25 patients testing positive for Shigella. The ipaH and ipaBCD genes were detected in almost all isolates and, unsurprisingly, all Shigella isolates associated with colitis were able to invade HeLa cells. This work alerts for multiple antibiotic resistant Shigella in the region and characterizes presence of ipa virulence genes and invasion phenotypesin dysenteric shigellosis.
Documentos Relacionados
- Frequency of serovars and antimicrobial resistance in Shigella spp. from Brazil
- Malaria control in an agro-industrial settlement of Rondônia (Western Amazon Region, Brazil)
- Identification, antimicrobial resistance and genotypic characterization of enterococcus spp. isolated in Porto Alegre, Brazil
- Etiology of diarrheal infections in children of Porto Velho (Rondonia, Western Amazon region, Brazil)
- PCR for Detection of Shigella spp. in Mayonnaise