Characterization of sequence elements at the 5' end of a discoidin I gene isolated from Dictyostelium discoideum.
AUTOR(ES)
Devine, J M
RESUMO
The discoidin I genes of Dictyostelium discoideum encode a family of three closely related developmentally regulated lectins which may play a role in cell cohesion. We have isolated a genomic clone containing the 5' half of a discoidin I gene with 12kb of flanking sequence and we have identified the discoidin I polypeptide encoded by this gene. We have determined part of the nucleotide sequence of the cloned segment and have hence determined the N-terminal amino acid sequence of the encoded polypeptide. The nucleotide sequence upstream of the AUG initiation codon was unusually AT rich, with 94% AT base pairs in the 171 nucleotides for which sequence was determined. However, having determined the start point of transcription ('cap' site) two striking features of similarity with other eukaryotic structural genes became apparent. The sequence TATAAA was present 33 nucleotides upstream from the 'cap' site and the sequence CCAAT was present a further 20 nucleotides upstream.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=320521Documentos Relacionados
- Unusual nucleotide sequences at the 5' end of actin genes in Dictyostelium discoideum.
- Genomic instability and mobile genetic elements in regions surrounding two discoidin I genes of Dictyostelium discoideum.
- Specific regulation of transcription of the discoidin gene family in Dictyostelium discoideum.
- Triphosphate residues at the 5' ends of rRNA precursor and 5S RNA from Dictyostelium discoideum.
- Overproduction of discoidin I by a temperature-sensitive motility mutant of Dictyostelium discoideum.
