Characterization of physicochemical forces involved in adhesion of Listeria monocytogenes to surfaces.

AUTOR(ES)

Mafu, A A

RESUMO

This study investigated the physicochemical forces involving the adhesion of Listeria monocytogenes to surfaces. A total of 22 strains of L. monocytogenes were compared for relative surface hydrophobicity with the salt aggregation test. Cell surface charges and hydrophobicity of L. monocytogenes Scott A were also determined by electrophoretic mobility, hydrophobic-interaction chromatography, and contact angle measurements. Electrokinetic measurements indicated that the strain Scott A has a negative electrophoretic mobility. Physicochemical characterization of L. monocytogenes by various methods indicates that this microorganism is hydrophilic. All L. monocytogenes strains tested with the salt aggregation test method aggregated a at very high ammonium sulfate molarities. The hydrophobicity-interaction chromatography results show that L. monocytogenes Scott A cells do not adhere to octyl-Sepharose unless the pH is low. Results from contact angle measurements showed that the surface free energy of strain Scott A was 65.9 mJ.m-2, classifying this microorganism as a hydrophilic bacterium. In addition, the interfacial free energy of adhesion of L. monocytogenes Scott A estimated for polypropylene and rubber was lower than that for glass and stainless steel. However, these theoretical implications could not be correlated with the attachment capabilities of L. monocytogenes.

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