Characterization of chicken c-ski oncogene products expressed by retrovirus vectors.
AUTOR(ES)
Sutrave, P
RESUMO
We constructed replication-competent avian retrovirus vectors that contain two of the three known types of chicken c-ski cDNAs and a third vector that contains a truncated c-ski cDNA. We developed antisera that recognize the c-ski proteins made by the three transforming c-ski viruses. All three proteins (apparent molecular masses, 50, 60, and 90 kilodaltons) are localized primarily in the nucleus. The proteins are differentially phosphorylated; immunofluorescence also suggests that there are differences in subnuclear localization of the c-ski proteins and that c-ski protein is associated with condensed chromatin in dividing cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=360678Documentos Relacionados
- Activation of the c-ski oncogene by overexpression.
- Isolation and characterization of three distinct cDNAs for the chicken c-ski gene.
- C-ski cDNAs are encoded by eight exons, six of which are closely linked within the chicken genome.
- The v-ski oncogene encodes a truncated set of c-ski coding exons with limited sequence and structural relatedness to v-myc.
- Interaction with Smad4 Is Indispensable for Suppression of BMP Signaling by c-Ski