Characterization of Cfa1, a Monofunctional Acyl Carrier Protein Involved in the Biosynthesis of the Phytotoxin Coronatine

AUTOR(ES)

Seidle, Heather

FONTE

American Society for Microbiology

RESUMO

Cfa1 was overproduced in Escherichia coli and Pseudomonas syringae, and the degree of 4′-phosphopantetheinylation was determined. The malonyl-coenzyme A:acyl carrier protein transacylase (FabD) of P. syringae was overproduced and shown to catalyze malonylation of Cfa1, suggesting that FabD plays a role in coronatine biosynthesis. Highly purified Cfa1 did not exhibit self-malonylation activity.

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