Characterization of an Ultraviolet B-Induced Lipase in Arabidopsis1
AUTOR(ES)
Lo, Maisie
FONTE
American Society of Plant Biologists
RESUMO
An Arabidopsis expressed sequence tag clone, 221D24, encoding a lipase has been characterized using an antisense approach. The lipase gene is expressed during normal growth and development of Arabidopsis rosette leaves but is down-regulated as the leaves senesce. When plants are exposed to sublethal levels of UV-B radiation, expression of the lipase is strongly up-regulated. The lipase protein is localized in the cell cytosol and is present in all organs of Arabidopsis plants. Recombinant lipase protein produced in Escherichia coli preferentially hydrolyzed phospholipids, indicating that the gene encodes a phospholipase. Transgenic plants in which lipase expression is suppressed showed enhanced tolerance to UV-B stress but not osmotic stress and were unable to up-regulate PR-1 expression when irradiated with UV-B. The observations collectively indicate that the lipase is capable of deesterifying membrane phospholipids and is up-regulated in response to UV-B irradiation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=514129Documentos Relacionados
- Cell membrane is a major locus for ultraviolet B-induced alterations in accessory cells.
- Erythropoietin Concentration in Amphotericin B-Induced Anemia
- Dynamic changes in and time sequence of ultraviolet B-induced apoptosis in rat corneal epithelial cells
- Delphinidin suppresses ultraviolet B-induced cyclooxygenases-2 expression through inhibition of MAPKK4 and PI-3 kinase
- Mechanisms of amphotericin B-induced decrease in glomerular filtration rate in rats.
