Characterization of an Equine Infectious Anemia Antigen Extracted from Infected Horse Spleen Tissue
AUTOR(ES)
Norcross, N. L.
RESUMO
The spleens of horses infected with equine infectious anemia contain an antigen that is useful for a diagnostic immunodiffusion test. This antigen was extracted from the spleen by homogenization of the tissue, centrifugation, and precipitation from the supernatant fluid at 50% saturation with (NH4)2SO4. The antigen was purified by subjecting it to two cycles of electrophoresis in a continuous free-flow electrophoresis cell and finally filtering through a column of Sephadex G-200 gel. The antigen was found to be a small protein with a molecular weight of 27,500 and sedimentation coefficient of 2.1S. Its density was about 1.18 and its isoelectric point 5.8. At 45 to 50 C, it coagulated, losing its antigenicity. The antigen was useful for assaying antibody in the serum from infected horses by using the complement fixation test or the immunodiffusion test. Complement-fixing antibodies were found to be more transient than the precipitating antibodies.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=416346Documentos Relacionados
- Demonstration of Antigenic Identity Between Purified Equine Infectious Anemia Virus and an Antigen Extracted from Infected Horse Spleen
- Equine Infectious Anemia Virus from Infected Horse Serum
- Detection of proviral DNA in horse cells infected with equine infectious anemia virus.
- Characterization of RNA from equine infectious anemia virus.
- Purification of equine infectious anemia virus antigen by affinity chromatography.
