Characterization of a presecretory phase in B-cell differentiation.
AUTOR(ES)
King, L B
RESUMO
We have identified and characterized an inducible in vitro subclone of the CH12 B-cell lymphoma, CH12-LBK, which appears to represent a transitional phase in the B-cell differentiation pathway. This phase, which we call the "presecretory" phase, falls between replicating B cells that are not secreting antibodies and B cells that secrete antibody at a high rate. Presecretory cells are characterized by abundant steady-state levels of immunoglobulin and joining (J) chain transcripts and of protein but low levels of mouse mammary tumor virus envelope transcripts and low rates of immunoglobulin secretion. Additional stimulation is required for presecretory cells to differentiate into cells that secrete antibodies at a high rate. The existence of cells with this phenotype suggests that high-level expression of immunoglobulin and J-chain protein does not necessarily commit a B cell to polymerize and secrete multimeric immunoglobulin. Rather, other gene products, expressed after immunoglobulin and J-chain transcripts have been upregulated late in B-cell differentiation, appear responsible for inducing high rates of antibody secretion.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=287009Documentos Relacionados
- Molecular definition of the germinal centre stage of B-cell differentiation.
- Sequential induction of NF-kappa B/Rel family proteins during B-cell terminal differentiation.
- Qualitative changes in the subunit composition of kappa B-binding complexes during murine B-cell differentiation.
- Human CD100, a novel leukocyte semaphorin that promotes B-cell aggregation and differentiation.
- Interleukin-5 (IL-5) and IL-6 define two molecularly distinct pathways of B-cell differentiation.