Characterization of a Na+/H+ antiporter gene of Escherichia coli.

AUTOR(ES)

Goldberg, E B

RESUMO

A mutant of Escherichia coli with increased Na+/H+ antiport activity was isolated and found by other workers to harbor two mutations [Niiya, S., Yamasaki, K., Wilson, T.H. & Tsuchiya, T. (1982) J. Biol. Chem. 257, 8902-8906]. The mutation that leads to increased Na+/H+ antiport (antup) has now been separated and mapped. antup maps in the vicinity of 0.5 min on the E. coli map, and the presence of this mutation alone in an isogenic pair raises antiport activity (Vmax) by approximately 4-fold. We also characterized cells hearing plasmids containing fragments of a 15-kilobase-pair segment of DNA between carA and dnaJ. A wild-type gene located within 2 kilobase pairs counterclockwise of rpsT increases the Na+/H+ antiport activity when present in multiple copies.

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