Characterization of a monoclonal antibody that selectively recognizes a subset of Entamoeba histolytica isolates.
AUTOR(ES)
Bhattacharya, A
RESUMO
Monoclonal antibody 2D7.10 recognized an antigen present in seven of nine isolates of axenically cultured Entamoeba histolytica and absent in all other Entamoeba isolates studied. The antigen was absent in two isolates: 200:NIH and Rahman. All nine isolates belonged to pathogenic zymodeme II. Western blot (immunoblot) analysis and treatment with periodate and the proteolytic enzyme trypsin suggest that the antigen recognized by 2D7.10 is a carbohydrate moiety.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=313678Documentos Relacionados
- A monoclonal antibody for distinction of invasive and noninvasive clinical isolates of Entamoeba histolytica.
- Molecular and cellular characterization of the 29-kilodalton peripheral membrane protein of Entamoeba histolytica: differentiation between pathogenic and nonpathogenic isolates.
- Preparation of Recombinant Human Monoclonal Antibody Fab Fragments Specific for Entamoeba histolytica
- Bacterial Expression of a Human Monoclonal Antibody-Alkaline Phosphatase Conjugate Specific for Entamoeba histolytica
- Characterization of a monoclonal antibody that defines an immunoregulatory T cell subset for immunoglobulin synthesis in humans.