Células tronco mesenquimais: diferenciação osteogênica in vitro estimulada pela atividade física in vivo com e sem bloqueio de óxido nitrico sintase e sua aplicação no tratamento local de osteoporose de ratas

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Three experiments were performed. The experiment 1 evaluated the effect of the physical activity with and without the nitric oxide synthase inhibition in the bone histomorfometry of rats with osteoporosis. The experiment 2 evaluated the osteogenic differentiation in vitro of mesenchymal stem cells (MSCs) of rats with osteoporosis submitted to the physical activity with and without nitric oxide synthase inhibition. The experiment 3 evaluated the effect of the intra-bone injection of differentiated MSCs in vitro in the treatment of the femoral osteoporosis of rats. It was conclude that the nitric oxide acts as mediator of the effects of the physical activity on the bone of rats with osteoporosis. MSCs of the rats with osteoporosis presented smaller osteogenic differentiation. The physical activity increased the osteogenic differentiation of MSCs of rats with osteoporosis. The nitric oxide synthase inhibition had negative effect in the osteogenic differentiation of MSCs of normal rats and with osteoporosis submitted to the physical activity, but it increased the osteogenic differentiation of MSCs of rats with sedentary osteoporosis. The treatment of the femoral osteoporosis with differentiated MSCs promoted increase the trabecular bone percentage for values similar to the control group in the whole femur

ASSUNTO(S)

veterinária teses osteoporose teses células tronco teses rato como animal de laboratório teses

ACESSO AO ARTIGO

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