Cell lines established by a temperature-sensitive simian virus 40 large-T-antigen gene are growth restricted at the nonpermissive temperature.
AUTOR(ES)
Jat, P S
RESUMO
The thermolabile large T antigen, encoded by the simian virus 40 early-region mutant tsA58, was used to establish clonal cell lines derived from rat embryo fibroblasts. These cell lines grew continuously at the permissive temperature but upon shift-up to the nonpermissive temperature showed rapidly arrested growth. The growth arrest occurred in either the G1 or G2 phase of the cell cycle. After growth arrest, the cells remained metabolically active as assayed by general protein synthesis and the ability to exclude trypan blue. The inability of these cell lines to divide at the nonpermissive temperature was not readily complemented by the exogenous introduction of other nuclear oncogenes. This finding suggests that either these genes establish cells via different pathways or that immortalization by one oncogene results in a finely balanced cellular state which cannot be adequately complemented by another establishment gene.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=362586Documentos Relacionados
- Functional characterization of temperature-sensitive mutants of simian virus 40 large T antigen.
- Oligomerization of simian virus 40 large T antigen is not necessarily repressed by temperature-sensitive A gene lesions.
- Temperature-sensitive mutants identify crucial structural regions of simian virus 40 large T antigen.
- Topoisomerase I Associates Specifically with Simian Virus 40 Large-T-Antigen Double Hexamer–Origin Complexes
- Analysis of a large-T-antigen variant expressed in simian virus 40-transformed mouse cell line mKS-A.