Cell-Free Synthesis of Encephalomyocarditis Virus

AUTOR(ES)

Svitkin, Yuri V.

FONTE

American Society for Microbiology

RESUMO

We developed a system for complete replication of encephalomyocarditis virus (EMCV) in a test tube by using an in vitro translation extract from Krebs-2 cells. Efficient virus synthesis occurred in a narrow range of Mg2+ and EMCV RNA concentrations. Excess input RNA impaired RNA replication and virus production but not translation. This suggests the existence of a negative-feedback mechanism for regulation of RNA replication by the viral plus-strand RNA or proteins.

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