cDNA isolation and gene expression of the maize annexins p33 and p35.

AUTOR(ES)

Battey, N H

RESUMO

The isolation, cloning, and sequencing of two full-length cDNAs corresponding to the root tip forms of the maize (Zea mays L. cv Clipper) annexins p33 and p35 are described. These are the first complete sequences for the widely reported doublet of plant annexins. The predicted sequences can be divided into four repeat domains characteristic of the annexin family, but Ca2+ binding by the type-II site typical of annexins would be predicted to occur only in repeats 1 and 4. This reduced number of sites is consistent with previously reported biochemical data indicating a high Ca2+ requirement for membrane association. Although the two annexins are very similar (80% amino acid identity), their genes are quite distinct, as demonstrated by their different 3' noncoding regions and Southern blotting. The predicted sequences of the root tip proteins are very similar to regions known from peptide sequencing of the coleoptile proteins. Because a rather small gene family is indicated, the implication is that there may be less functional diversity than in animal cells. Furthermore, the sequence data clearly show that plant annexins form a very distinct group compared with those from other kingdoms.

Documentos Relacionados

• Isolation of cDNA clones for the p33 invariant chain associated with HLA-DR antigens.
• Isolation and characterization of a human p53 cDNA clone: expression of the human p53 gene.
• Biotin synthase from Arabidopsis thaliana. cDNA isolation and characterization of gene expression.
• Uncoupled Expression of p33 and p92 Permits Amplification of Tomato Bushy Stunt Virus RNAs
• Isolation and sequence comparison of a maize calmodulin cDNA.