Caracterização molecular do vírus da hepatite c em indivíduos co-infectados com HIV-1

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Human immunodeficiency virus (HIV-1) and Hepatitis C virus (HCV) are considered problems of public health with about 40 millions infected by HIV-1 and 170 millions infected by HCV worldwide. The co-infection HIV-1/HCV is common in individuals exposed to percutaneous pathways and the hepatitis C has been emerging as the main cause of death in HIV-1 positive individuals due the high prevalence of HCV in this population. The HCV is classified into 6 genotypes and several subtypes. Differences in the genotypic distribution are observed in different areas worldwide and within a country. Some HCV genotypes appear to be related to a better virological response after treatment, as the genotypes 2 and 3 for example, that show better response than individuals infected with genotypes 1 and 4. There is little information about co-infected HIV-1/HCV population; the genotype prevalence is reported to the infections separately. Thus, the aim of this study was to characterize the HCV genotypes and subtypes prevalence in the co-infected HIV-1/HCV population of Federal District. Forty-five samples from co-infected patients were analyzed by PCR amplification of 5‟UTR and NS5B genomic sequences followed by automatic sequencing and phylogenetic analysis. The HCV genotype 1 was the most prevalent (81%), followed by genotype 3 (10%), 2 (6%) and 4 (3%). This result is in concordance with reports from other studies from Central West region of Brazil, where there is a major prevalence of genotype 1. The genotypes 2 and 4, rare in Brazil, were described for the first time in the HCV positive population of Federal District. The agreement between the results obtained by the analysis of the sequences homology by the software HCV-BLAST to the regions 5‟UTR and NS5B was of 97% for genotypes and of 90% for subtypes, corroborating with the described in literature as to the need to the analyze more than one genomic region in order to achieve correct subtype determination. The phylogenetic analysis defined the diverging genotypes and subtypes. The presence of the genotypes 1, 2, 3, and 4 in the co-infected HIV-1/HCV population attended in the Federal District indicates the importance of genotyping in this population once these genotypes have clinical importance in the prediction of the response to the antiviral treatment.

ASSUNTO(S)

fígado - doenças hepatite c hiv-1 biologia molecular

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