Caracterização e purificação da enzima bromelina em sistema de duas fases aquosas PEG/Fosfato / Bromelain enzyme caracterization and purification using two aqueous phases system PEG/Phosphate

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

The bromelains are proteases from vegetable origin, obtained from several species of Bromeliaceae family, which are found in pineapple s stem and fruit. The bromelains uses are based on its proteolytic activity on food industries and mostly at Brazilian pharmaceutical industry. The bromelains has been documented by its therapeutic effects at all inflammatory conditions, in addition to its attested efficiency of cure of various health problems, such as angina, indigestion and respiratory problems. Various bromelains purification methods has been studied, particularly of stem s bromelain. Aqueous two-phase systems has been applied for several years as a good laboratorial separation technique and they can be used as a prepurification step. These systems have been tested with large success. The systems are formed by two aqueous phases immiscible or partially miscible within themselves, obtained by the addition of hydrophilic polymers or one hydrophilic polymer and a salt, such as the poly(ethylene glycol) -PEG - and potassium phosphate salt systems. This work studied the enzyme characterization and recuperation presented in the pineapple s stem and skin, by aqueous two-phase systems liquid-liquid extraction. Batch assays were performed aiming the enzyme extraction and recuperation, using the partition coefficient as indicator. The phase diagrams from PEG and potassium phosphate salts aqueous two-phase systems were obtained for PEG molecular weight of 1500 and 4000 at pH 6.0 to 11.0, at 25 ºC. Three different tie-lines were also studied. The pH and tieline length influence on the enzyme partition coefficient were analysed

ASSUNTO(S)

liquid extraction extração quimica bromelain benzimas - purificação pinneapple purification two aqueous phases system abacaxi bromelina

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