Caracterização de um begomovírus obtido de maracujazeiro (Passiflora edulis f. flavicarpa) e produção de clones infecciosos / Characterization of a begomovirus obtained from yellow passionfruit (Passiflora edulis f. flavicarpa) and production of infectious clones
AUTOR(ES)
Sávio de Siqueira Ferreira
DATA DE PUBLICAÇÃO
2008
RESUMO
Begomoviruses (family Geminiviridae) possess a circular, single-stranded DNA genome encapsidate in twinned icosahedral particles. They are vectored by the whitefly Bemisia tabaci and cause economically important diseases in several crops, mainly in tropical and subtropical regions. In Brazil, begomoviruses are major pathogens in common bean and tomato crops, and recent reports have confirmed their presence in yellow passionfruit. In spite of the low incidence, their importance in this crop should not be underestimated, due to the severity of the disease and the plants inability to recover from the infection. The objective of this work was to carry out the molecular and biological characterization of the begomovirus isolate BR:LNS2:Pas:01, obtained from yellow passionfruit plants in Livramento de Nossa Senhora, Bahia state. The viral genome (DNA-A and DNA-B) was cloned and sequenced. Sequence analysis demonstrated that the BR:LNS2:Pas:01 DNA-A has five ORFs corresponding to the genes cp, rep, trap, ren and ac4, and two additional ORFs with high sequence identity with the AC5 ORF. The DNA-B has two ORFs corresponding to the genes mp and nsp. The DNA-A of isolate BR:LNS2:Pas:01 has the highest nucleotide sequence identity with Tomato chlorotic mottle virus (ToCMoV) (77%), and the DNA-B with Tomato yellow spot virus (ToYSV) (74%). These identity values indicate that this isolate represents a new begomovirus species, for which the name Passion flower severe leaf distortion virus (PSLDV) is proposed. Phylogenetic analysis clustered the PSLDV DNA-A in a monophyletic branch with TGMV, and the DNA-B in a monophyletic branch with ToYSV. Biological characterization consisted in the determination of the isolates host range, via biolistic inoculation of infectious clones in plants of several species/cultivars. The host range was restricted to species from the Passifloraceae and Solanaceae families. However, in spite of the high sequence identity between PSLDV-[BR:LNS2:Pas: 01] and tomato begomoviruses, the virus was not capable of infecting tomato.
ASSUNTO(S)
biologia molecular clones begomovírus maracujá begomovirus yellow passionfruit clones
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