Caracterização da pureza de fosfatidilcolina da soja através de RMN de ¹H e de 31P
AUTOR(ES)
Mertins, Omar, Sebben, Marcelo, Henrique Schneider, Paulo, Pohlmann, Adriana Raffin, Silveira, Nádya Pesce da
FONTE
Química Nova
DATA DE PUBLICAÇÃO
2008
RESUMO
A strategy is proposed to evaluate the purity of phosphatidylcholine from soybean lecithin, obtained by extraction or column chromatography, using the integrals ratio of ¹H NMR spectra. Integrals of methylene signals, around 1.3 and 1.6 ppm, are added and divided by the integral of the choline methyl groups, around 3.3 ppm. Before purification, a ratio of 19.68±1.37 was determined. Using extraction, a ratio of 10.70±0.61 was found, while from column chromatography, a value of 2.99±0.25 was detected. 31P NMR of standard phosphatidylcholine showed signals at -0.2 and -0.9 ppm, whereas the purified one showed a single signal at -0.9 ppm.
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