Caracterização bioquímica e molecular de isolados microbianos presentes em solo contaminado por petróleo

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

27/10/2009

RESUMO

The bioremediation processes are becoming increasingly sought strategies related to soil contamination by oil. To achieve this goal, one good alternative is the isolation of indigenous microorganisms from the impacted region, to study and to know the native species, their diversity and metabolism, and then use the best microbial specie as inoculum on bioremediation processes. This study aimed to obtain isolates of the microbial community from an oil contaminated soil, and identify the isolates, as well as to perform the biochemical characterization of the isolates with potential ability to use oil as a carbon source. The isolates able to degrade crude oil showed good lipase activity (0,100 U/mL, 0,211 U/mL and 0,948 U/mL), and biosurfactant biosynthesis with an emulsification index of 9.935 %, 3.546 % and 26.087 %, respectively. In addition, the biosurfactants produced suffered small variation on the stability index when assayed against pH changes and temperature variations. The molecular diversity analys was based on RAPD markers, and subsequent identification of isolates by the partial sequencing of the 16S rRNA gene. A high molecular diversity could be observed among the isolates, and 8 different bacterial genera could be identified. The isolates with higher potential of use as bioremediation agents were identified as belonging to the species Bacillus subtilis

ASSUNTO(S)

petróleo - biodegradação solos - remediação biorremediação solos - poluição por petróleo soil remediation bioremediation petroleum biodeterioration oil pollution of soils

ACESSO AO ARTIGO

Documentos Relacionados