Caracterização biologica e morfologica da linhagem paulista de Schistosoma mansoni da região de Campinas, SP

AUTOR(ES)
DATA DE PUBLICAÇÃO

1994

RESUMO

The São Paulo State ( Brazil ) shows autochtonous schistosomiasis mansom in several regions, including the region of Campinas. This suggests a possible development of this disease during the last three years. The present work was elaborate having in view to determine biological characteristics of Schistosoma mansoni recently isolated trom the Ward São Domingos, municipality of Campinas, SP. The isolation was camed out in 1993, originating of hatched miracidia trom eggs of feces of three autochthonous patients and non-treated to schistosomiasis. The study was developed using as experimental model swiss mice-sympatric Biomphalaria tenagophila. The suscetibility of 180 snails B. tenagophila exposed to 1 and 0 miracidia was evaluated. Eighty mice were infected with 70 cercariae by immersion of the tail in water. Afier 10 weeks, they were killed and evaluated folIowing this analysis: suscetibility of S. mansoni to oxamniquine ( 100 mg/Kg, single dose, oral route ) and to praziquantel ( 100 mg/Kg, 5 days, oral route ); ability of cercariae penetration and worm recovery; body weights, of tiver and spleen; mortality rate ; diameter of hepatic granuloma and morphometry of adults woms and eggs. The mortality rates until 77 days of infection was 58,3% ( 1 miracidium ) and 93,3% ( 10 miracidia ), indicating that the infection was significant to the death of the animals. The rate of infection were low: 8,3<1ó to 10 miracidia and 3,30/0 to 1 miracidium. This date indicates that the strain CAMP-H is not well-adapted to its intermediate host The experimental chemotherapy revealed tha1 praziquantel (95,4%) was lightly more effective than oxamniquine (94,4%), while the percentage of surviving worms was of 11,9% to the first drug and of 17% to the second one. This percentages were statistically different and it was considered high. This finding suggest that new studies wiIl be necessary to determine the oviposition of surviving worms of treated mice. We also verified high rate of cercariae penetration ( 98,1% ) and of recovery worms ( 53,3% ) in the infected mice; aIteration of the ponderal growth from infected animaIs succeding ina decrease of the weight when compared to the controI group; in the period between the administration of the two antischistosomal drugs and the killing of the animaIs, we noticed body weight gain of treated animals; hepatomegaly (3 :i: 0,56g ) and spIenomegaly ( 0,82 :i: 0,23g ) showing a weight gain of 72,8<}ó and 1000/0, respectiveIy, when compared to these viscera of the controI group; granulomatous reactions hepatic measuring 296,08 :i: 81,27 J.1.m2, The measurements of worms was of 6,15 :i: 1,47mm for male and 5,02 :i: 1,22mm for female; and the eggs showed 56,37 :i: 8,09J.1.m of width and 133,69 :i: 19,98J.1.m of Iength. The S. mansoni strain of Campinas ( SP ) showed different characteristics from other strains of São Paulo State as SJ ( Paraíba Valley ), Itariri ( Ribeira Valley ), Ourinhos (Paranapanema Valley) and ofBH strain (Belo Horizonte, Minas Gerais State)

ASSUNTO(S)

parasito esquistossome mansonica - campinas (sp)

ACESSO AO ARTIGO

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