Caracterização biológica e molecular do vírus da mancha clorótica de Clerodendrum ( Clerodendrum Chlorotic Spot Virus-CLCSV) / Biological and molecular characterization of Clerodendrum chlorotic spot virus

AUTOR(ES)

Renata Takassugui Gomes

DATA DE PUBLICAÇÃO

2009

RESUMO

The botanical genus Clerodendrum belongs to the family Lamiaceae and includes several ornamental species, usually climbing, and heart-bloody (C. x speciosum Tiejism. &Binn.) and tear-in-Christ (C. thomsonae Balf. ) are among the most cultivated. Necrotic and chlorotic spots on leaves of heart-blood have been observed for the first time in a garden of Piracicaba, associated with an infestation of Brevipalpus phoenicis Geijskes (Acari: Tenuipalpidae). Sections of diseased tissues examined in the electron microscope revealed characteristic cytopathic effects of the nuclear type and concluded that the symptoms were caused by a virus transmitted by Brevipalpus (VTB), tentatively named Clerodendrum chlorotic spot virus (ClCSV). This virus is transmitted through mechanical inoculation from heart-bloody to heart-bloody and in preliminary tests mechanically and by mites for several other plants, in addition to the natural occurrence of its spread to other species. Systemic infection occurs in the host Chenopodium quinoa Will. and C. amaranticolor Coste &Reyn. infected with ClCSV if the plants are kept at 28-30°C for about 2 weeks. These plants were used for partial purification of vírus. This study presents the biological and molecular characterization of ClCSV. Through the PTA-ELISA and RT-PCR tests was possible to detect the ClCSV in different host, in addition to the analysis of serological and molecular reaction of this virus with other type of nuclear VTBs. From the sequencing of the PCR product was obtained from nucleotide sequences that showed similarity to the polymerase of OFV (Orchid Fleck Virus), another type of nuclear VTB. Morpho-anatomical analysis were performed to see the changes caused by ClCSV in tissues of Clerodendrum x speciosum and other infected hosts. It was possible to observe the occurrence of hypertrophy, cell plasmolisis and the reduction of starch grains in the áreas injured in all plants infected by ClCSV.

ASSUNTO(S)

histopathology. doenças de plantas clcsv viral genome vírus de plantas. Ácaros clerodendrum x speciosum brevipalpus phoenics histologia vegetal plantas ornamentais

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