CaracterizaÃÃo fenotÃpica, perfil de sensibilidade antifÃngica e estocagem de malassezia SSP / Phenotypic characterization, antifungal susceptibility profile and storage malassezia SSP

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

28/08/2008

RESUMO

The genus Malassezia enclose lipophylic and lipid-dependent yeast that after many changes in its taxonomic classification, comprises in the atuality 13 species, including M. pachydermatis, M. furfur, M. sympodialis, M. globosa, M. obtusa, M. restricta, M. slooffiae, M. dermatis, M. japonica, M. yamatoensis, M. nana, M. equine, and M. caprae. These yeasts are associated to several diseases including, such as pityriasis versicolor, or dermatoses, seborrheic dermatitis and atopic dermatitis, among others. The general aim of this study was to contribute to better knowledgement about the phenotypical identification, storage in fungal collection and in vitro antifungal susceptibility of Malassezia spp. The phenotiping was based on macro and micromorphologics characteristics, as well as biochemistry and nutritional analisys. Twenteen strains suffers storage at -80ÂC in vegetables oils. The microdilution technique was accomplished in RPMI 1640 broth, supplemented with ox bile, Tween 20 and glycerol, being complemented with subculture on Dixon agar, for determination of the minimal inhibitory concentration (MIC) and minimal fungicidal concentration (CFM). The drugs tested were ketoconazole (KET), itraconazole (ITR), fluconazole (FLC), amphotericin B (AMB) and caspofungine (CAS). With the conventional phenotypical analysis of the strains (n=38), could suggest the presence of the M. furfur/ M. dermatis (n=17), M. sympodialis (n=8), M. slooffiae (n=5) and M. pachydermatis (n=8). The stored accomplished in vegetable oils at -80ÂC showed meaningful rate of recorver, but physiologic characteristics was modified, such as _-glicosidase activity and Chremophor EL assimilation. Most of the strains (84,21%) was sensitive for KET and ITR, obtaining values of MIC 0.03μg/mL. For FLC the MIC range was 4 to 64μg/mL, against VOR the strains of M. pachydermatis obtained MIC range <0,03 a 2μg/mL, therefore the lipid-dependents strains of Malassezia spp. obtained dispersive results from <0.03 to >16μg/mL. Against ANB, the MIC range was 1 to >16μg/mL. It was not possible determinate the MIC and MFC values for caspofungine. The extracts from avocado seeds were active against strains of M. pachydermatis.

ASSUNTO(S)

microbiologia medica malassezia spp fenotipagem criopreservaÃÃo sementes sensibilidade antifÃngica malassezia spp., phenotyping, cryopreservation, seeds, antifungal suceptibility

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