Cacipacore virus as an emergent mosquito-borne Flavivirus

AUTOR(ES)

Figueiredo, Mario Luis Garcia de, Amarilla, Alberto Anastacio, Figueiredo, Glauciane Garcia de, Alfonso, Helda Liz, Lippi, Veronica, Maia, Felipe Gonçalves Motta, Morais, Felipe Alves, Costa, Cristóvão Alves da, Henriques, Dyana Alves, Durigon, Edison Luis, Figueiredo, Luiz Tadeu Moraes, Aquino, Victor Hugo

FONTE

Rev. Soc. Bras. Med. Trop.

DATA DE PUBLICAÇÃO

2017-08

RESUMO

Abstract INTRODUCTION: Cacipacore virus (CPCV), a possible bird-associated flavivirus, has yet to be detected in mosquitoes. Our purpose is examining CPCV in mosquitoes from the Amazon region of Brazil. METHODS: Approximately 3,253 Culicidae (grouped into 264 pools) were collected from the Amazon region during 2002-2006 and analyzed using a Flavivirus genus-specific reverse transcription- polymerase chain reaction followed by nested polymerase chain reaction assay and by nucleotide sequencing of amplicons. RESULTS: Nucleotide sequences from five mosquito samples showed high similarity to the those of CPCV originally isolated in the Amazon region. CONCLUSIONS: This is the first report of CPCV-infected mosquitoes which has implications on the arbovirus maintenance in nature and transmission to man.

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