Brewer's spent grain and corn steep liquor as alternative culture medium substrates for proteinase production by Streptomyces malaysiensis AMT-3
AUTOR(ES)
Nascimento, Rodrigo Pires do, Alves Junior, Nelson, Coelho, Rosalie Reed Rodrigues
FONTE
Braz. J. Microbiol.
DATA DE PUBLICAÇÃO
2011-12
RESUMO
Brewer's spent grain and corn steep liquor or yeast extract were used as the sole organic forms for proteinase production by Streptomyces malaysiensis in submerged fermentation. The influence of the C and N concentrations, as well as the incubation periods, were assessed. Eight proteolytic bands were detected through gelatin-gel-electrophoresis in the various extracts obtained from the different media and after different incubation periods, with apparent molecular masses of 20, 35, 43, 50, 70, 100, 116 and 212 kDa. The results obtained suggest an opportunity for exploring this alternative strategy for proteinases production by actinomycetes, using BSG and CSL as economically feasible substrates.
Documentos Relacionados
- Utilization of brewer’s spent grain and mushrooms in fortification of smoked sausages
- Production of red pigments by Monascus ruber in culture media containing corn steep liquor
- "A comparison between sugar consumption and ethanol production in wort by immobilized Saccharomyces Cerevisiae, Saccharomyces Ludwigii and Saccharomyces Rouxii on Brewer's Spent Grain"
- CORN STEEP LIQUOR IN MICROBIOLOGY
- Microbiological Aspects of Penicillin: IX. Cottonseed Meal as a Substitute for Corn Steep Liquor in Penicillin Production