Blood agar to detect virulence factors in tap water heterotrophic bacteria.
AUTOR(ES)
Payment, P
RESUMO
Cytolytic colonies were found in 57% of tap water samples, and up to 6% of samples were found to contain bacteria having three or more virulence factors. The factors evaluated were cytotoxicity, hemolysis, cell adherence, and cell invasiveness. Overall, 17% of the samples contained cytolytic colonies that were adherent and hemolytic. Among the media tested, tryptic soy agar with sheep blood (incubated at 35 degrees C for 48 h) was the best medium for the detection of cytolytic colonies. Of the colonies growing on this medium, 13% were cytolytic, whereas on medium R2A, less than 3% were cytolytic. Furthermore, when tryptic soy agar with blood was used, 24% of the samples contained colonies with at least three virulence factors whereas only 5% were positive with R2A. Routine monitoring by using tryptic soy agar with sheep blood is suggested as an appropriate procedure for the detection of bacteria with pathogenic potential in drinking water.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=201456Documentos Relacionados
- Enumeration of heterotrophic bacteria in water for dialysis: comparison of the efficiency of Reasoner'2 agar and plate count agar
- Chromogenic cephalosporin spot test to detect beta-lactamase in clinically significant bacteria.
- Effect of temperature on mineralization by heterotrophic bacteria.
- Evaluation of enrichment, storage, and age of blood agar medium in relation to its ability to support growth of anaerobic bacteria.
- Blood-lysing solution nontoxic to pathogenic bacteria.
