Biotyping of industrial yeasts by the Killer system. / Biotipagem de leveduras industriais através do sistema Killer.

AUTOR(ES)

Christiann Davis Tosta

DATA DE PUBLICAÇÃO

2004

RESUMO

Agriculture is an important sector in the economy of Brazil. The sugar cane occupies 9% of the cultivated area in this country. The most important products from the sugar cane industry are sugar and alcohol, the latest produced by fermentative process by yeasts. During the fermentation the yeast population changes due to the interferences coming from the sugar cane juice or other sources, turning the process susceptible to undesirable contaminations. In this way, fast, reliable and cheap methods for microbiological monitoring can be helpful. Selective culture media, biochemical tests and molecular analyses have been used but they are time-consuming or expensive. The killer phenomenon discovered initially in Saccharomyces cerevisiae have shown interesting results to yeast biotyping. The sensibility pattern to different killer toxins was used to make a “fingerprinting” and successfully separate different strains of yeasts. This method was corroborated by classical taxonomy and molecular biology results (PCR and RAPD-PCR). The results obtained gives support to development of a methodology useful on fermenting microbiologic monitoring with the selection of nine strains of killers yeasts with highly discrimination between industrial bakker yeasts and contaminants of the fermentation process.

ASSUNTO(S)

levedura industrial biologia molecular killer yeasts pcr reacao em cadeia por polimerase fingerprinting rapd-pcr microbiologia toxinas industria sucro-alcooleira biotyping yeats

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