Bioprospecção de Actinobactérias da rizosfera de milho ( Zea mays L.) com atividade antifúngica / Bioprospection of actinobacteria from maize (Zea mays L.) rhizosphere with antifungal activity.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Actinobacteria occur in different environments and have a great potential in producing enzymes, antibiotics and medical molecules. The present work aimed the isolation, identification and the bioprospection by rhizosphere actinobactérias. Sixty strains were isolated from healthy plants of maize, cultivated in different regions Socorro, Serra Negra and Ribeirão Preto. The two isolates with higher activity CCMA30 and CCMA33 were identified by the partial sequencing of the 16S rDNA gene as belonging to the species Streptomyces griseorubiginosus. In these isolates it was detected the presence of PKS genes. The fractioning of the crude extract of the strain CCMA 33 has revealed that fraction 7 (m/z 719-768) displayed antifungal activity and it was submitted to fragmentation (MS/MS), where by searches in the dictionary of natural products detected the presence of Julichromes Q6-6 and the presence of two other Julichromes (Q1-3 and Q3-3). These results highlight the production of secondary metabolites of biotechnological interest by rhizosphere actinobacteria.

ASSUNTO(S)

pks cromatografia metabólitos secundários bioprospection secondary metabolites chromatograph rhizosphere actinobacteria bioprospecção controle biológico actinobactérias rizosféricas biological control pks

ACESSO AO ARTIGO

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