CaracterizaÃÃo molecular (PCR) e infecÃÃo de Metarhizium anisopliae var. acridum e Metarhizium anisopliae var. anisopliae EM Zaprionus indianus / Molecular Characterization (PCR) and infection Metarhizium anisopliae var. acridum e Metarhizium anisopliae var. anisopliae EM Zaprionus indianus

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

The Metarhizium anisopliae var. acridum and M. anisopliae var. anisopliae strains were analysed for the pathogenicity to the fly Zaprionus indianus, using the concentrations 104, 105, 106, 107, 108 conidia/mL, considering the percentage of adultsâ emergency. In agreement with the used methodology, it was verified that both fungi strains presented action against Z. indianus. The ITS (Internal Trancribed Spacer) molecular markers of rDNA, Intron Splice Site Primer and Microsatelite (SSR- Simple Sequence Repeats) were used to evaluate the genetic diversity before and after passing through the fly. The grouping analysis using the UPGMA method, based on the genetic distances of molecular markers, confirmed the recognized genetic diversity of the genus Metarhizium. The microsatelite (GTG)5 and the introns of nuclear mRNA group showed the same sensitive to detect the genetic variability among Metarhizium strains. The amplification products of the rDNA locus ITS1-5.8-ITS2 with the ITS4 and ITS5 primers were efficient to demonstrate that the strains analysed belongs to the Metarhizium anisopliae specie, although genetic diversity was demonstrated by markers (GTG)5 e EI1. The amplification profiles of microsatelite, intron and ITS regions after passing through the Z. indianus proved that the reisolateds strains were the same that were used for infection

ASSUNTO(S)

regiÃo its-rdna microssatÃlite zaprionus indianus intron splice site primer metarhizium metarhizium its-rdna region microsatelite zaprionus indianus intron splice site primer micologia

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