Biological characterization of a myotoxin phosphoplipase A2 homologue purified from the venom of the snake Bothrops moojeni
AUTOR(ES)
Queiroz, MR, Mamede, CC, Fonseca, KC, Canabrava, LCMN, França, LV, Silva, MC, Stanziola, L, Beletti, ME, Canabrava, HAN, Oliveira, F
FONTE
Journal of Venomous Animals and Toxins including Tropical Diseases
DATA DE PUBLICAÇÃO
2011
RESUMO
A myotoxin phospholipase A2 homologue, BmooMtx, was isolated from the venom of Bothrops moojeni by a combination of ion-exchange chromatography on DEAE-Sephacel column and gel filtration on Sephadex G-75. SDS-PAGE showed the enzyme to be a monomer with a molecular weight of 16,500. BmooMtx induced release of creatine kinase and morphological analyses indicated that it provoked an intense myonecrosis, with visible leukocyte infiltrate and damaged muscle cells 24 hours after injection. Anti-BmooMTx antibodies partially neutralized the myotoxic activity of BmooMtx and crude B. moojeni venom, as judged by determination of plasma creatine kinase levels and histological evaluation of skeletal muscle in mice. Anti-BmooMTx antibodies were effective in reducing the plasma creatine kinase levels of crude B. alternatus and B. leucurus venoms, evidencing immunological cross-reactivity between BmooMTx and other bothropic venoms. Intraplantar (i.pl.) injection of BmooMtx (1 to 15 μg/animal) caused a dose- and time-dependent hyperalgesia and edematogenic responses. Dexamethasone (0.4 mg/kg), meloxicam (2 mg/kg) and promethazine (5 mg/kg) markedly reduced the hyperalgesia. Our data suggest that these drugs may likely serve as complementary therapies in cases of accidents with Bothrops moojeni, provided that such pharmacological treatments are administered immediately after the incident.
Documentos Relacionados
- Functional and Structural Characterization of an Acidic Toxic Phospholipase A2 from Bothrops moojeni Snake Venom.
- Physicoquemical and biologic characterization of phospholipase A2 isolated from Bothrops moojeni venom
- Functional and structural characterization of phospholipases A² isolated from Bothrops asper snake venom in Panamá
- Cytotoxic and pro-apoptotic action of MjTX-I, a phospholipase A2 isolated from Bothrops moojeni snake venom, towards leukemic cells
- Cytotoxic and inflammatory potential of a phospholipase A2 from Bothrops jararaca snake venom