Biological activities and phytochemical profile of Passiflora mucronata from the Brazilian restinga

AUTOR(ES)

Araujo, Marlon H. de, Silva, Isabel C.V. da, Oliveira, Pollyana F. de, Barreto, Arielly R.R., Konno, Tatiana U.P., Esteves, Francisco de A., Barth, Thiago, Aguiar, Fernando A., Lopes, Norberto P., Dermenjian, Renee K., Guimarães, Denise O., Leal, Ivana C.R., Lasunskaia, Elena B., Muzitano, Michelle Frazão

FONTE

Rev. bras. farmacogn.

DATA DE PUBLICAÇÃO

2017-12

RESUMO

ABSTRACT In general, Passiflora species have been reported for their folk medicinal use as sedative and anti-inflammatory. However, P. caerulea has already been reported to treat pulmonary diseases. Severe pulmonary tuberculosis, generally caused by Mycobacterium tuberculosis strains resistant to multiple drugs, can lead to deleterious inflammation and high mortality, encouraging new approaches in drug discovery. Thus, the aim of this work was to evaluate the Passiflora mucronata Lam., Passifloraceae, potential for tuberculosis treatment. Specifically, related to antimycobacterial activity and anti-inflammatory related effects (based on inhibition of nitric oxide, tumor necrosis factor-alpha production and antioxidant potential), as well as the chemical profile of P. mucronata. High performance liquid chromatography coupled with diode-array ultraviolet and mass spectrometer analyses of crude hydroalcoholic extract and ethyl acetate fraction showed the presence of flavonoids. Ethyl acetate fraction showed to be as antioxidant as Ginkgo biloba standard extract with EC50 of 14.61 ± 1.25 µg/ml. One major flavonoid isolated from ethyl acetate fraction was characterized as isoorientin. The hexane fraction and its main isolated compound, the triterpene β-amyrin, exhibited significant growth inhibitory activity against Mycobacterium bovis BCG (MIC50 1.61 ± 1.43 and 3.93 ± 1.05 µg/ml, respectively). In addition, Passiflora mucronata samples, specially hexane and dichloromethane fractions, as well as pure β-amyrin, showed a dose-related inhibition of lipopolysaccharide (LPS)-induced nitric oxide production. In conclusion, Passiflora mucronata presented relevant biological potential and should be considered for further studies using in vivo pulmonary tuberculosis model.

Documentos Relacionados

• Phytochemical Profile of Pasture Weeds from the Brazilian Cerrado
• Effects that nutritional and saline gradients have on the growth of Passiflora mucronata Lam. and Canavalia rosea (Sw.) DC. found in the restinga of Brazil
• Biological activities of marine invertebrates extracts from the northeast brazilian coast
• Aspectos histológicos da minienxertia de Passiflora edulis sims. e Passiflora mucronata lam.
• Morphophysiology, Phenotypic and Molecular Diversity of Auxin-induced Passiflora mucronata Lam. (Passifloraceae)