Bioconversion from crude glycerin by Xanthomonas campestris 2103: xanthan production and characterization
AUTOR(ES)
Brandão, L. V., Assis, D. J., López, J. A., Espiridião, M. C. A., Echevarria, E. M., Druzian, J. I.
FONTE
Braz. J. Chem. Eng.
DATA DE PUBLICAÇÃO
2013-12
RESUMO
The production and rheological properties of xanthan gum from crude glycerin fermentation, a primary by-product of the biodiesel industry with environmental and health risks, were evaluated. Batch fermentations (28 °C/250 rpm /120 h) were carried out using crude glycerin, 0.01% urea and 0.1% KH2PO4, (% w/v), and compared to a sucrose control under the same operational conditions, using Xanthomonas campestris strain 2103 isolate from Brazil. Its maximal production by crude glycerin fermentation was 7.23±0.1 g·L-1 at 120 h, with an apparent viscosity of 642.57 mPa·s, (2 % w/v, 25 °C, 25 s-1), 70% and 30% higher than from sucrose fermentation, respectively. Its molecular weight varied from 28.2 to 36.2×10(6) Da. The Ostwald-de-Waele model parameters (K and n) indicated a pseudoplastic behavior at all concentrations (0.5 to 2.0 %, w/v) and temperatures (25-85 °C), while its consistency index indicated promising rheological properties for drilling fluid applications. Therefore, crude glycerin has potential as a cost-effective and alternative substrate for non-food grade xanthan production.
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